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With solar radiation being a primary driver of melting glacial ice and snow, glaciers and high-elevation mountain snowpacks are especially sensitive to even small changes in the concentration of light absorbing particles. Surface melt of snow and glacial ice is substantially higher if impurities such as mineral dust and organic matter are present in significant quantities. Bacteria and algae further promote darkening of the glacial surface and melting by aggregating these impurities in the form of biofilm. Like many mountain glaciers of the Alaskan region, the Juneau Icefield has seen extensive mass loss. Black carbon released by human and natural activities has become a major contributor to reducing snow and ice albedo. Microbes can affect the dynamics of black carbon on glacial surfaces, with biodegradation having profound implications on its residence time, light absorbance, and output to adjacent and downstream aquatic ecosystems. This NSF Rapid Response Research (RAPID) project funded the field work necessary for the acquisition of samples from the Gilkey Glacier, Alaska in July 2024. This dataset includes sample collection types, coordinates and stream flow data. 

ABSTRACT We report the genomic sequences of 14 bacterial isolates from a supraglacial stream on the Cotton Glacier, Antarctica. Fine sediments in the streambed provide habitat for bacterial growth and biofilm formation. The stream represents a natural laboratory for studying the evolution and adaptation of microbes to a humic-free environment. 

ABSTRACT Inland meltwater ponds are common throughout the dry valley region of Antarctica, with seasonal meltwater inputs driving their biogeochemistry. Here, we report the genomic sequences of eight environmental bacterial isolates covering three major phyla from Marr Pond, Taylor Valley, Antarctica. 

ABSTRACT Sediments in cryoconite holes and meltwater streams in the McMurdo Dry Valleys, Antarctica, provide both substrates and conditions that support life in an arid polar desert. Here, we report the genomic sequences of eight environmental, bacterial isolates from Canada Glacier cryoconite holes and stream. These isolates span three major phyla. 

Abstract Environmentally relevant metagenomes and BONCAT-FACS derived translationally active metagenomes from Powder River Basin coal seams were investigated to elucidate potential genes and functional groups involved in hydrocarbon degradation to methane in coal seams with high- and low-sulfate levels. An advanced subsurface environmental sampler allowed the establishment of coal-associated microbial communities under in situ conditions for metagenomic analyses from environmental and translationally active populations. Metagenomic sequencing demonstrated that biosurfactants, aerobic dioxygenases, and anaerobic phenol degradation pathways were present in active populations across the sampled coal seams. In particular, results suggested the importance of anaerobic degradation pathways under high-sulfate conditions with an emphasis on fumarate addition. Under low-sulfate conditions, a mixture of both aerobic and anaerobic pathways was observed but with a predominance of aerobic dioxygenases. The putative low-molecular-weight biosurfactant, lichysein, appeared to play a more important role compared to rhamnolipids. The methods used in this study—subsurface environmental samplers in combination with metagenomic sequencing of both total and translationally active metagenomes—offer a deeper and environmentally relevant perspective on community genetic potential from coal seams poised at different redox conditions broadening the understanding of degradation strategies for subsurface carbon. 

ABSTRACT Auxotrophy, or an organism's requirement for an exogenous source of an organic molecule, is widespread throughout species and ecosystems. Auxotrophy can result in obligate interactions between organisms, influencing ecosystem structure and community composition. We explore how auxotrophy-induced interactions between aquatic microorganisms affect microbial community structure and stability. While some studies have documented auxotrophy in aquatic microorganisms, these studies are not widespread, and we therefore do not know the full extent of auxotrophic interactions in aquatic environments. Current theoretical and experimental work suggests that auxotrophy links microbial community members through a complex web of metabolic dependencies. We discuss the proposed ways in which auxotrophy may enhance or undermine the stability of aquatic microbial communities, highlighting areas where our limited understanding of these interactions prevents us from being able to predict the ecological implications of auxotrophy. Finally, we examine an example of auxotrophy in harmful algal blooms to place this often theoretical discussion in a field context where auxotrophy may have implications for the development and robustness of algal bloom communities. We seek to draw attention to the relationship between auxotrophy and community stability in an effort to encourage further field and theoretical work that explores the underlying principles of microbial interactions. 

As many bacteria detected in Antarctic environments are neither true psychrophiles nor endemic species, their proliferation in spite of environmental extremes gives rise to genome adaptations. Janthinobacterium sp. CG23_2 is a bacterial isolate from the Cotton Glacier stream, Antarctica. To understand how Janthinobacterium sp. CG23_2 has adapted to its environment, we investigated its genomic traits in comparison to genomes of 35 published Janthinobacterium species. While we hypothesized that genome shrinkage and specialization to narrow ecological niches would be energetically favorable for dwelling in an ephemeral Antarctic stream, the genome of Janthinobacterium sp. CG23_2 was on average 1.7 ± 0.6 Mb larger and predicted 1411 ± 499 more coding sequences compared to the other Janthinobacterium spp. Putatively identified horizontal gene transfer events contributed 0.92 Mb to the genome size expansion of Janthinobacterium sp. CG23_2. Genes with high copy numbers in the species-specific accessory genome of Janthinobacterium sp. CG23_2 were associated with environmental sensing, locomotion, response and transcriptional regulation, stress response, and mobile elements—functional categories which also showed molecular adaptation to cold. Our data suggest that genome plasticity and the abundant complementary genes for sensing and responding to the extracellular environment supported the adaptation of Janthinobacterium sp. CG23_2 to this extreme environment. 

Abstract Steep, boulder bed streams often contain sediment patches, which are areas of the bed with relatively well‐defined boundaries that are occupied by distinct grain size distributions (GSD). In sediment mixtures, the underlying GSD affects the critical Shields stress for a given grain size, which is commonly predicted using hiding functions. Hiding functions may vary with reach‐wide bed GSD, but the effect of local GSD on relative sediment mobility between sediment patches is poorly understood. We explore the effects of patch‐scale GSD on sediment mobility using tracer particles combined with local shear stresses to develop hiding functions for different patch classes within a steep stream. Hiding functions for all tested patch classes were similar, which indicates that the same hiding function can be used for different patches. However, the critical Shields stress for a given grain size generally decreased with lower patch median grain size (D₅₀) suggesting that patches control the relative mobility of each size through both the underlying GSD and local shear stresses. The effects of the underlying GSD partly depend on grain protrusion, which we measured for all grain sizes present on each patch class. Protrusion was generally greater for larger grains regardless of patch class, but for a given grain size, protrusion was increased with smaller patchD₅₀. For a given grain size, higher protrusion results in greater applied fluid forces and reduced resisting forces to partly explain our lower critical Shields stresses in finer patches. Patches therefore can importantly modulate relative sediment mobility through bed structure and may need to be included in reach‐scale sediment transport and channel stability estimates. 

Here, we present the draft genome sequence for the violacein-producing Janthinobacterium sp. CG23_2 isolated from an Antarctic supraglacial stream. The genome is ~7.85 Mb, with a G+C content of 63.5%. The genome includes 7,247 candidate protein coding genes, which may provide insight into UV tolerance mechanisms.